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KMID : 0380019960110030311
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Korean Journal of Biotechnology and Bioengineering
1996 Volume.11 No. 3 p.311 ~ p.316
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Effects of Polyethylene Glycol on Glucoamylase Production in Recombinant Yeast Culture
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Cha Hyung-Joon
Yoo Young-Je
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Abstract
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The effects of polyethylene glycol (PEG) on glucoamylase production in recombinant Saccharomyces cerevisiae were studied. By PEG addition, the cell growth was not affected. However, the glucoamylase production was increased in all range of PEG molecular weights and concentrations. The optimal molecular weight of PEG was 6000 and the optimal concentration was 1g/L. Using these optimals, the extracellular glucoamylase activity was 23% higher in PEG-containing medium than that in medium without PEG.
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